MICROPROPAGATION OF ROSA DAMASCENA MILL.: THE EFFECTS OF GELLING AGENTS ON THE MULTIPLICATION STAGES AND ACCLIMATIZATION

Doina Clapa, Alexandru Fira, Orsolya Borsai, Monica Hârța, Cristian Sisea, Rodica Pop, Doru Pamfil

Abstract


The aim of our research was to investigate the way in which the agar types used for culture media preparation influence damask rose in vitro proliferation rates, as well as the quality of the shoots obtained and their ability for ex vitro rooting and acclimatization. For this purpose, seven types of gelling agents were tested: 6 g/L Daishin Agar, 4 g/L Gelcarin, 2 g/L Gelrite, 15 g/L Isubgol, 4 g/L Micro Agar, 6 g/L Phyto Agar and 4 g/L Plant Agar. The culture media consisted of Murashige & Skoog supplemented with 4 mg/L 6-benzyladenine. The highest multiplication rate, 5.1, was obtained on the culture media supplemented with 6g/L Daishin Agar and 4 g/L Plant Agar and the lowest one was 2.8, obtained on the media gelled with 4 g/L Gelcarin. Although the multiplication rate on the media gelled with Isubgol was lower (3.2), the shoots obtained in this experimental treatment got acclimatized with 90 % survival percentage and developed the best root system, regarding the number of roots/shoot and root length.


Keywords


Daishin Agar, Gelcarin, Gelrite, Isubgol, Micro Agar, Phyto Agar, Plant Agar



DOI: http://dx.doi.org/10.15835/agrisp.v103i3-4.12830