Liquid chromatographic method for the determination of beta-carotene from milk and cheese
Abstract
The beta-carotene content in milk and cheese is an accurate indicator for the feeding method used for cows. It can also be sued as an indicator of adulteration of goat and other types of milk with cow milk. A high accuracy HPLC-UV method for the determination of beta-carotene from milk and cheese has been developed. After saponification with an alcoholic solution of potassium hydroxide the samples where extracted with a mixture of hexane: ethyl acetate (70:30) was used. The concentrated extract was analyzed using Perkin Elmer 200 Series High Performance Liquid Chromatograph (HPLC) with UV detector for the determination of beta-carotene content. The calibration was done in 4 points from 1-20 ng/ml. The recovery yield was between 73,8-89,7 %. The method was tested on 3 milk samples and 5 cheese samples. The highest concentration of beta-carotene was found in cheddar cheese produced with milk from pasture raised cows.
Copyright (c) 2020 Anca Becze, Mihai O. Naghiu, Vanda L. Babalau Fuss
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The papers published in the journal are licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Open Access Journal: The journal allow the author(s) to retain publishing rights without restrictions. Authors are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under the Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
