LC/MS/MS METHOD DEVELOPMENT FOR IDENTIFICATION AND QUANTIFICATION OF 17Β-ESTRADIOL IN WASTE WATER

  • Dorina Simedru (Rusu) INCDO-INOE 2000, Research Institute for Analytical Instrumentation - ICIA, Cluj-Napoca
  • Anca Naghiu INCDO-INOE 2000, Research Institute for Analytical Instrumentation - ICIA, Cluj-Napoca

Abstract

Many chemicals which are released into the environment and have the potential to disrupt the function of endocrine systems are called endocrine disrupting chemicals. A category included in these EDCs is the estrogens. A significant representative of this category is 17β-estradiol which is excreted by both male and female. Its presence in waste water is very dangerous because, following the path of waste water; it goes to rivers causing significant damage to fish environment. Due to its importance, a method was developed for screening and confirming 17β-estradiol residue in waste water samples. Method development consisted in preparing the standard solutions, optimizing the compound and source dependent parameters, choosing the mobile phase and the appropriate HPLC column followed by processing a calibration curve and finally injecting a real sample. The waste water sample was extracted according to some bibliographic studies, then injected on a Phenomenex Luna C18 column and detected through a LC/MS/MS system in an ESI (electrospray ionization) negative mode. The results were considered to be satisfying.
Published
2010-08-14
Section
Research articles