EXPRESSION ANALYSIS OF SOME BOILING STABLE PROTEINS (HYDROPHILINS) UNDER COMBINED EFFECT OF DROUGHT STRESS AND HEAT SHOCK IN DROUGHT TOLERANT AND SUSCEPTIBLE CULTIVARS OF TRITICUM AESTIVUM

Gurmeen Rakhra, Arun Dev Sharma

Abstract


The combined effect of drought stress and heat shock on the induction of boiling stable proteins viz: WGA, SOD, HSP90,Aquaporin, CyPs, APase and LEA proteins was studied in 3-days old seedlings of drought tolerant and drought susceptible cultivars of wheat. Boiling stable protein profile was outlined via SDS electrophoresis of tissue extracts. The results obtained were confirmed by Immunoblot analysis with anti-WGA, anti-SOD, anti-HSP90, anti-APase, anti-Aqua, anti-Cyp and anti-LEA antibodies. Western blot analysis revealed the induction of boiling stable proteins (SOD, HSP90, Aquaporin, CyPs) during combined drought and heat stress (DH) conditions as compared to separately applied heat (H) and drought treatments (D) in drought tolerant cultivars of wheat, indicating their role in water stress adaptation under simultaneous applied abiotic stress conditions. Alternation in boiling stable protein expression was more pronounced in seeds as compared to shoots of both the cultivars. Based upon these observations the possible role of hydrophilins in water stress tolerance is discussed.

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DOI: http://dx.doi.org/10.15835/arspa.v81i1-2.7882



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