ANTIOXIDANT ACTIVITY OF CAROTENOIDS EXTRACTS FROM HIPPOPHAE RHAMNOIDES

Andreea Stanila, S. Stanila

Abstract


This work aimed to verify carotenoids antioxidant activity in raw sunflower oil, oxidized with Cu2+ ion. In order to evaluate this activity it was used standards of β-carotene, luthein and total carotenoids extract from Hippophae rhamnoides (seabuckthorn). Sunflower oil was treated with CuSO4 solution 100mM necessary to induce peroxidation and antioxidant activity of carotenoids was evaluated by decreasing of their amount, measured at 445nm after 1h and 500C heating, 24h and 500C, 48h and 500C . The best antioxidant activity had luthein at higher concentration. Comparing with luthein, seabuckthorn extract has similar antioxidant activity with this standard. Peroxidation level was established by MDA-TBA test, using spectrofluorimetric method.

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DOI: http://dx.doi.org/10.15835/arspa.v79i3-4.8068



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