The molecular polymorphism evaluation in Salix sp. Romanian accessions - preliminary results
Abstract
Salix is an important energetic genus, but  most of the genotypes are closely related genetically, due to frequent natural hybridisation in nature. Therefore it is of great interest to study its genetic background. In this work a collection of progenitors (Romanian accessions) collected from different locations were investigated to determine their genetic fingerprint in order to be used in future breeding programs. To evaluate the genetic diversity two types of molecular markers were used, namely ISSR (Inter Simple Sequence Repeats) and  DAMD (Direct amplification of minisatellite-region). In the first step 15 ISSR and 5 DAMD markers were analysed to choose those markers that give the best amplification. It was pointed out that all of the primers had complex fingerprints, but those  which generated the most definite patterns were A13 and UBC 818 (ISSR) and 1-URP6R and 4- 14C2 (DAMD).These primers were used to analyze seven different Salix L. samples collected from different locations. It was revealed that a high polymorphism was identified both between different accesions of a species and between species. The high degree of polymorphism for both  minisatellite and microsatellite sequences, emphasized the necessity of increasing markers number to be possible to perform a statistical analysis and to determine the genetic similarities.
Authors who publish with this journal agree to the following terms:
a) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).