Arbuscular Mycorrhizae Morphotype-Based Identification Using Optical Microscopy

Ioana CRIŞAN, Roxana VIDICAN, Vlad S TOIAN

Abstract


Structures of different AM taxa found in symbiosis with plants can stain from intense to weak after roots preparation for microscopy. Although arbuscules are distinctive features of AM, their overall shape can vary in association with two proliferation types: Arum and Paris. Auxiliary cells and vesicles are not omnipresent. Intraradical hyphae can present divergent types: straight/coiled, with “Y”/“H” connections. According to spore formation, there are five main AM morphotypes. Spore-based species identification takes into account: color, shape, size of spores and sporocarps as well as structures associated with them (cicatrix, pedicel, peridium, sporiferous saccule, sporogenous cell, subtending hypha). Description of subcellular structures of spores targets phenotypic and biochemical properties of layers using Melzer’s reagent and morphology of pre-germination structures when present.

Keywords


colonization pattern; hyphae; arbuscules; spores; vesicles

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DOI: http://dx.doi.org/10.15835/buasvmcn-hort:2018.0026


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