Optimization of ITEX/GC-MS Method for Determination of Indigenous Rosemary Volatiles
Abstract
Volatile compounds from rosemary dried leaves samples were determined using in-tube extraction technique coupled with GC-MS analysis (ITEX/GC-MS). In order to achieve the best results, the extraction parameters (incubation time, incubation temperature, sample weight, number of strokes) were optimized. Depending on the selected extraction parameters, a number of 7 to 23 volatiles were separated. The major volatile constituents of rosemary dried leaves were α-pinene, eucalyptol, 3-octanone, limonene, camphene, camphor. The larger number of extracted compounds was obtained at high incubation temperatures, but also in the case of an increased number of strokes.Authors who publish with this journal agree to the following terms:
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