Evaluation of Physical-Chemical Indexes, Sugars, Pigments and Phenolic Compounds of Fruits from Three Apple Varieties at the End of Storage Period

Andruta Elena Muresan, Sevastita Muste, Andrei Borsa, Romina Alina Vlaic, Vlad Muresan


Apples are the most cultivated and consumed fruits in the world. They not only taste great, but there are also rich sources of monosaccharides, pigments, fibers, functional compounds such as polyphenols which are well-known for their antioxidant action. Due to the high level of apples consumption, it is important to monitor and know the detailed chemical composition of this fruits on the market shelf. The aim of this paper was to study the detailed chemical composition of apples from three varieties. Samples from three varieties (Ionathan, Golden Delicious and Starkrimson) were taken from the Romanian market. Individual sugars composition was performed by HPLC, total polyphenols content by Folin Ciocalteu method, antioxidant capacity by using the DPPH test, while pigments were analysed by spectrophotometric specific methods and the total starch content measured by a polarimetric method. Water content, acidity, total soluble solids and pH were also monitored through specific methods. There were found differences between varieties particularly in relation to the polyphenols content, carotenoids and chlorophyll. Regarding the individual sugars composition, fructose and glucose were predominant followed by sucrose for all samples. Values of starch, moisture, acidity, total soluble solids and the pH were according to other apple varieties found in literature. These results provide important information regarding the chemical composition of apple varieties from Romanian market, for both human direct consumption and industrial processing.



Ionathan, Golden Delicious, Starkrimson, apple chemical composition, sugars, pigments, antioxidants.

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Arnon, D.I. (1949). Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. PlantPhysiol, 24:1–15.

Bunea, A., D.O. Rugină, A.M. Pintea, Z. Sconţa, C.I. Bunea, C. Socaciu (2011). Comparative polyphenolic content and antioxidant activities of some wild and cultivated blueberries from Romania. Not Bot Horti Agrobo, 39:70-76.

Bunea, A., M. Andjelkovic, C. Socaciu, O. Bobis, M. Neacsu, R.verhe, J. V. Camp (2008). Total and individual carotenoids and phenolic acids content in fresh, refrigerated and processed spinach (Spinacia oleracea L.). Food Chemistry, 649–656.

Britton, G., S. Liaaen-Jensen, H. Pfander (1995) Carotenoids. Birkhauser Verlag: Basel, Swtzerland, Vol.1A, 1B

Cerbu, E.A., S. Muste, A. Borşa, Z. Sconţa, D. Crainic, V. Mureşan (2012). Total phenolic content changes during apple growth as a function of variety and fruit position in the crown. Journal of Agroalimentary Processes and Technologies, 18: 341-344.

Cerbu, E.A., S. Muste, A. BorÅŸa, M. Simona, V. MureÅŸan (2011). Relationship between the Polarimetric Determination of Starch and the Starch Iodine Index in Apple Variety Jonathan, Bulletin UASVM Agriculture, 68:533.

Campeanu, G., G. Neata, G. Darjanschi (2009). Chemical composition of the fruits of several apple cultivars growth as biological crop. Not. Bot. Hort. Agrobot. Cluj 37:161-164.

Drogoudi, P., Z. Michailidis, and G. Pantelidis (2008). Peel and flesh antioxidant content and harvest quality characteristics of seven apple cultivars. Scientia Horticulturae 115:149-1

Henriquez C., S. Almonacid, I. Chiffelle, T. Valenzuela, M. Araya, L. Cabezas, R. Simpson, and H. Speisky (2010). Determination of antioxidant capacity, total phenolic content and mineral composition of different fruit tissue of five apple cultivars grown in chile. Chilean Journal of Agricultural Research 70:523-536.

Zheng, H. (2012) A profile of physicochemical and antioxidant changes during fruit growth for the utilisation of unripe apples. Food Chemistry, 131:106–110.

Lancaster, J., E. Jan, E. Grant, C. E., Lister (1994). Skin Color in Apples—Influence of Copigmentation and Plastid Pigments on Shadeand Darkness of Red Color in Five Genotypes. J. Amer. Soc. Hort. Sci. 119:63-69.

Lizabethlister C. (1994). Biochemistry of fruitcolour in apples (Malus pumilaMill.). University of Canterbury, Christchurch, New Zealand.

Miller, N. J., C. A. Rice-Evans (1997). The relative contributions of ascorbic acid and phenolic antioxidants to the total antioxidant activity of orange and apple fruit juices and blackcurrant drink. Food Chemistry, 60:331–337.

Muste S. (2008). Materii prime vegetale în industria alimentară. Editura AcademicPres, Cluj-Napoca.

Odriozola-Serrano I., R. Soliva-Fortuny, O. Marti (2008). Effect of minimal processing on bioactive compoundsand color attributes of fresh-cuttomatoes. Science Direct, LWT, 41:217–226.

Rosnah, S., W. K., Wong, M. Noraziahand, H. Osman (2012). Chemical composition changes of two water apple (Syzygium samaragense). International Food Research Journal 19:167-174.

Sconţa, Z. M. (2012). Extraction, Purification, Characterization and in vitro testing of anthocyanin-rich fractions obtained from aronia melanocarpa and vaccinium Sp., PhD Thesis, Cluj-Napoca, România.

Sestras A., R. Sestras, V. Lazar, V. Mitre, I. Mitre, G. Ropan, A. Barbos (2009). The influence of fruit position in the crown of trees on the sugar content and morphological traits of apple fruits, Bulletin UASVM Horticulture, 66.

Jihong W., H. Gao, L. Zhao, X. Liao, F. Chen, Z. Wang, H. Xiaosong (2007). Chemical compositional characterization of some apple cultivars. Food Chemistry 103:88–93.

*** FAO (2013). FAOSTAT - FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS. http://faostat.fao.org/site/567/DesktopDefault.aspx?PageID=567#ancor (23.11.2013)

*** HARMONISEDMETHODS OF THE INTERNATIONAL HONEYCOMMISSION (2002). IHC responsible for the methods: Stefan Bogdanov Swiss Bee Research Centre FAM, Liebefeld, CH-3003 Bern, Switzerland.

*** AOAC (1999). Official methods of analysis. 16th ed. 5th revision. Volume II. Chapter 44. Subchapter 4. Cunnif, P. (ed.) AOAC International, Washington, D.C., USA.

*** ISO (1997). International Standard, ISO 10520, Native starch – Determination of starch content – Ewers polarimetric method.

*** ISO (1998). Uganda Standard, US ISO 750, Fruit and vegetable products - Determination of titratable acidity.

*** ISO (2003). International Standard, ISO 2173, Fruit and vegetable products Determination of soluble solids - Refractometric method.

DOI: http://dx.doi.org/10.15835/buasvmcn-fst:10111


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