Evaluation of Antioxidant Activity and Phenolic Content in Different Salvia officinalis L. Extracts

Ana Viorica Pop (Cuceu), Maria Tofană, Sonia A. Socaci, Dan Vârban, Melinda Nagy, Maria DoiniÅ£a Borș, Susana Sfechiș

Abstract


The use of medicinal plants to improve health is an ancient practice and in recent years it has been observed an increasing interest of scientific researchers for the study of plants with biological properties and active principles responsible for their therapeutic effects. Salvia officinalis L. is considered the queen of herbs and belongs to the Lamiaceae (Labiatae) family. Due to the increasing interest in plants health benefits, the aim of the present study was to characterize various extracts of Romanian sage regarding their content in compounds with antioxidant activity. Three different techniques and five solvents were used for extraction of bioactive compounds from Salvia officinalis L. The total phenolic content and the antioxidant activity of plant extract were determined by Folin-Ciocalteu method and respectively by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Methanolic extract exhibited the highest content in phenolic compound (1974.89 mg GAE/100g dw) as well ass the strongest antioxidant capacity (85.12%).


Keywords


antioxidant capacity, extraction, phenolic content, Salvia officinalis

Full Text:

PDF

References


Alam MN, Bristi NJ, Rafiquzzaman M (2013). Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal 21:143–152.

Amirmohammadi M, Khajoenia S, Bahmani M, Rafieian-Kopaei M, Eftekhari Z, Qorbani M (2014). In vivo evaluation of antiparasitic effects of Artemisia abrotanum and Salvia officinalis extracts on Syphacia obvelata, Aspiculoris tetrapetra and Hymenolepis nana parasites. Asian Pac J Trop Dis 4(1): 250-254.

Balouiri M, Sadiki M, Ouedrhiri W, Farah A, El Abed S, Koraichi SI (2014). Antibacterial activity of extracts from Salvia officinalis and Rosmarinus officinalis obtained by sonication and maceration methods. Int J Pharm Pharm Sci 6(2):167-170.

Bettaieb I, Hamrouni-Sellami I, Bourgou S, Limam F, Marzouk B (2011). Drought effects on polyphenol composition and antioxidant activities in aerial parts of Salvia officinalis L. Acta Physiol Plant 33:1103–1111.

Danesi F, Kroon PA, Saha S, de Biase D, D’Antuono LF, Bordoni A (2014). Mixed pro- and anti-oxidative effects of pomegranate polyphenols in cultured cells. Int J Mol Sci 15(11):19458–19471.

Dragovic-Uzelać V, Garofulić IE, Jukić M, Penić M, Dent M (2012). The influence of microwave-assisted extraction on the isolation of sage (Salvia officinalis L.) polyphenols. Food Technol. Biotechnol. 50(3):377–383.

Gomar A, Hosseini A, Mirazi N (2014). Evaluation of Salvia officinalis L. (sage) leaves on morphine-induced memory impairment in adult male rats. Focus on Alternative and Complementary Therapies 19(3):156–162.

Martins N, Barros L, Santos-Buelgam C, Henriques M, Silva S, Ferreira ICFR (2015). Evaluation of bioactive properties and phenolic compounds in different extracts prepared from Salvia officinalis L. Food Chemistry 170:378–385.

Murugan R, Parimelazhagan T (2014). Comparative evaluation of different extraction methods for antioxidant and anti-inflammatory properties from Osbeckia parvifolia Arn. – An in vitro approach. Journal of King Saud University – Science 26:267–275.

Odrizola-Serrano I, Soliva-Fortuny R, Martin-Belloso O (2008). Effect of minimal processing on bioactive compounds and color attributes of fresh-cut tomatoes. LWT-Food Science and Technology 41:217-226.

Oliveira KB, Palú E, Weffort-Santos AM, Oliveira BH (2013). Influence of rosmarinic acid and Salvia officinalis extracts on melanogenesis of B16F10 cells. Rev. Bras. Farmacogn. Braz. J. Pharmacogn 23(2): 249-258.

Pizzale L, Bortolomeazzi R, Vichi S, Uberegger E, Conte LS (2002). Antioxidant activity of sage (Salvia officinalis and S fruticosa) and oregano (Origanum onites and O indercedens) extracts related to their phenolic compound content. J Sci Food Agric 82:1645–1651.

Quiroz-Reyes CN, Aguilar-Mendez MA, Ramirez-Ortız ME, Ronquillo-De Jesus E (2013). Comparative study of ultrasound and maceration techniques for the extraction of polyphenols from cocoa beans (Theobroma cacao L.). Revista Mexicana de Ingenierıa Quimica 12(1):11-18.

Roby MHH, Sarhana MA, Selima KAH, Khalel KI (2013). Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts. Industrial Crops and Products 43:827– 831.

Shamnas M, Kumar R, Teotia UVS (2014). Antioxidant and anti-inflammatory activity of methanol extract of Salvia officinalis flowers. American Journal of PharmTech Research 4(2):166-175.

Skotti E, Anastasaki E, Kanellou G, Polissiou M, Tarantilis PA (2014). Total phenolic content, antioxidant activity and toxicity of aqueous extracts from selected Greek medicinal and aromatic plants. Industrial Crops and Products 53:46– 54.

Socaci SA, Socaciu C, Mureşan C, Fărcaş A,Tofană M, Vicaş S, Pintea A, (2013). Chemometric discrimination of different tomato cultivars based on their volatile fingerprint in relation to lycopene and total phenolics content. Phytochem. Anal. 25:161–169.

Soran ML, Cobzac SC, Varodi C, Lung I, Surducan E, Surducan V (2009). The extraction and chromatographic determination of the essentials oils from Ocimum basilicum L. by different techniques. Journal of Physics: Conference Series 182.

VeliÄković DT, Karabegović IT, StojiÄević SS, Lazić ML, Marinković VD, Veljković VB (2011). Comparison of antioxidant and antimicrobial activities of extracts obtained from Salvia glutinosa L. and Salvia officinalis L. Hem. Ind. 65(5):599–605.




DOI: http://dx.doi.org/10.15835/buasvmcn-fst:11582

Refbacks

  • There are currently no refbacks.



University of Agricultural Sciences and Veterinary Medicine
3-5 Manastur St., 400372 Cluj-Napoca
Romania
Tel: +40-264-596.384 | Fax: +40-264-593.792 
biroupublicatii@usamvcluj.ro