Comparative Antifungal Effect of Lactic Acid Bacteria Strains on Penicillium digitatum

Adrian Matei, Călina PetruÈ›a Cornea, Sorin Matei, Gabi Mirela Matei, Steliana Rodino


Lactic acid bacteria (LAB) are natural alternative to chemical preservatives for fruits. The aim of the research was to select LAB strains with high antifungal activity against Penicillium digitatum for the biopreservation of fruits. The antifungal activity of eight lactic acid bacteria strains has been evaluated against Penicilliuum digitatum isolated from orange, by overlay assay method and by optical microscope examination. The reversion of inhibition zone after 96 h was recorded as a fungistatic effect while those with inhibition zone for at least 7 days were recorded as fungicidal. The antifungal effect of efficient LAB strains was assessed by comparing inhibition of fungal biofilm formation in liquid media. The strains Lpl, Lpa, LAB 13, LAB 15, LAB 43 and LAB 58 presented intense antifungal activity with clear inhibition zones diameter over 20 mm. The microscopy evidenced atypical hyphae and delaying of conidial chain formation. The strains Lpa, LAB 13, LAB 15 fully inhibited the mycelia growth, strains LAB 43 and LAB 58 partly with delaying of biofilm formation on the surface of culture medium. The results of comparative antifungal activity of LAB strains evidenced the highest inhibition of fungal biofilm formation and structural damages of hyphae and spores caused by the strains Lpa, LAB 13 and LAB 15. These strains could be efficient biocontrol agents of Penicillium digitatum in fruits.


Penicillium digitatum, antifungal activity, biofilm, lactic acid bacteria, overlay assay

Full Text:



Adebayo CO, Aderiye BI (2010). Antifungal activity of bacteriocins of lactic acid bacteria from some Nigerian fermented foods. Res. J. Microbiol., 5:1070-1082.

Blagojev N, Skrinjar M, Veskovic-Moracanin S, Soso V (2012). Control of mould growth and mycotoxin production by lactic acid bacteria metabolites. Rom. Biotechnol. Lett., 17:7219-7226.

Bryden WL (2007). Mycotoxin in the food chain: Human health implications. Asia Pacific J. Clin. Nutr., 16, 95-101.

Bryden WL (2012). Mycotoxin contamination of the feed supply chain: Implications for animal productivity and feed security. Animal Feed and Sci. Technol., 173:134-158.

Dalie DKD, Deschamps AM, Richard-Forget F (2010). Lactic acid bacteria – Potential for control of mould growth and mycotoxins: A review. Food Control, 21:370-380.

El-Deeb N, Sharaf M, El-Adawi H (2015). Antibacterial and plasmid curing activity of lactic acid bacteria against multidrug resistant bacteria strains. Int. J. Pharmacol. 11(2):114-121.

Erginskaya Z, Ãœnal E, Kalkan S (2011). Importance of microbial antagonism about food attribution. In: Science against microbial pathogens: communicating current research and technological advances. A. Mendez-Villas (Ed.), 1342-1348.

Hamed HA, Moustafa YA, Abdel-Aziz SM (2011). In vivo efficacy of lactic acid bacteria in biological control against Fusarium oxysporum for protection of tomato plant. Life Sci. J., 8(4): 462-468.

Laref N, Guessas B (2013). Antifungal activity of newly isolates of lactic acid bacteria. Inov. Romanian Food Biotechnol., 13:80-88.

Magnusson J, Strom K, Roos S, Schnurer J (2003). Broad and complex antifungal activity among environmental isolates of lactic acid bacteria. FEMS Microbiol. Let., 219:129-135.

Masood MI, Qadir MI, Shirazi JH, Khan IU (2011). Beneficial effects of lactic acid bacteria on human beings. Crit. Rev. Microbiol., 37(1):91-98.

Schillinger U, Villarreal JV (2010). Inhibition of Penicillium nordicum in MRS medium by lactic acid bacteria isolated from foods. Food Control, 21:107-111.

Trias R, Baneras L, Montesinos E, Badosa E (2008). Lactic acid bacteria from fresh fruit and vegetables as biocontrol agensts of phytopathogenic bacteria and fungi. Int. Microbiol., 11:231-236.

Ãœnal E, Kalkan S, Erginkaya Z (2011). Use of lactic acid bacteria biofilms as biocontrol agents. Sci. Technol. Against Microbial Pathogens, 207-209.

U.S Department of Health and Human Services, Food and Drug Administration Center for Food Safety and Applied Nutrition, 2014.



  • There are currently no refbacks.

University of Agricultural Sciences and Veterinary Medicine
3-5 Manastur St., 400372 Cluj-Napoca
Tel: +40-264-596.384 | Fax: +40-264-593.792