High-purity Anthocyanins Isolation using Solid Phase Extraction Tehniques

Zorița Diaconeasa, Dumitrița Rugină, Carmen Socaciu


Anthocyanins are water soluble pigments used as natural colorants which have been extendedly studied due to their beautiful colors, and more important, beneficial health potential. Published studies regarding anthocyanins health benefits have been performed using crude anthocyanin extracts from natural sources with no any data about the purity of the tested extract. The impurities from the crude extracts are mostly other phenolic compounds; which can also have biological proprieties. In this case the beneficial potential of crude extract is not attributed only to anthocyanin. Thus, data regarding anthocyanins purity are required for the biological research. Currently, most investigations on anthocyanins are focused on their stability, as well as their purification and identification. In this paper, the most recent techniques in the chemical investigation of the anthocyanins purification are summarized.


anthocyanins, purification, SPE

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Ahmadiani Neda (2012). Anthocyanin Based Blue Colorants. The Ohio State University

Andersen OM, Jordheim M (2006). The anthocyanins. In: Andersen O.M., Markham K.R. (Eds): Flavonoids Chemistry, Biochemistry and Applications. CRC Press, Taylor and Francis, Boca Raton: 471–551.

Bunea A, Rugină D, Sconţa Z, Pop R, Pintea A, Socaciu C, Tăbăran F, Struijs K, Van Camp J (2013). Anthocyanins determination in various cultivars of highbush blueberries and their antiproliferation and apoptosis properties in B16-F10 metastatic murine melanoma cells, Phytochemistry, 95:436-44

Brouillard R (1982). Chemical Structure of Anthocyanins. In: Anthocyanins as Food Colors, Markakis, P. (Ed.). Academic Press, New York, London, Paris, pp: 1-40.

Welch Cara R, Wu Q and James ES (2008). Recent Advances in Anthocyanin Analysis and Characterization, Curr Anal Chem, 4(2): 75–101.

Castaneda-Ovando A, Pacheco-Hernandez MDL, Paez-Hernandez ME, Rodriguez JA, Galan Vidal CA (2009). Chemical studies of anthocyanins: A review. Food Chem; 113:859-871.

Clifford MN (2000). Anthocyanins – nature, occurrence and dietary burden. Journal of the Science of Food and Agriculture, 80(7)1063–1072.

Diaconeasa Z, Leopold L, Rugină D, Ayvaz H, Socaciu C (2015). Antiproliferative and antioxidant properties of anthocyanin rich extracts from blueberry and blackcurrant juice. Int J Mol Sci, 16:2352-65.

Vargas DF, Jimenez AR, Paredes-Lopez O (2000). Natural pigments: carotenoids, anthocyanins, and betalains--characteristics, biosynthesis, processing, and stability, Crit Rev Food Sci Nutr 40:173.

Hou De-Xing (2009). Nutrigenomics and Proteomics in Health and Disease: Food Factors and Gene Interactions, Chapter 16. Cancer-Preventive Effects and Molecular Actions of Anthocyanins, edited by Yoshinori Mine, Kazuo Miyashita, Fereidoon Shahid

Dey PM, Harborne JB, (Eds) 1993. Plant phenolics. Methods in plant biochemistry (2nd printing), Academic, London

Bailon Escribano MT, Santos-Buelga C (2003). Polyphenols Extraction from Foods.in: Methods in Polyphenol Analysis (eds.C. Santos-Buelga, G. Williamson). Royal Society of Chemistry, Cambridge, United Kingdom, pp. 1–16.

Fossen T, Slimestad R, Ovstedal D, Andersen OM (2002). Biochem Syst Ecol 30:855.

Takeoka G And Lan D (2002). Chapter 5. Anthocyanins, Methods of Analysis for Functional Foods and Nutraceuticals, Edited by W . Jeffrey Hurst CRC Press Print ISBN: 978-1-56676-824-5 eBook ISBN: 978-1-4200-1467-9.

Giusti MM, 2007. Analisys of anthocyanins Food Colorants Chemical and Functional Properties, 429–547, ISBN: 978-0-8493-9357-0

Giusti MM, Wrolstad RE (2003). Acylated anthocyanins from edible sources and their applications in food systems. Biochemical Engineering Journal 14: 217-225.

Harborne JB and Harborne AJ (1998). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Kluwer Academic Publishers, London, UK

He J and Giusti MM (2011). High-purity isolation of anthocyanin mixtures from fruits and vegetables – a novel solid-phase extraction method using mixed mode cation-exchange chromatography.Journal of Chromatography, A 1218:7914-7922

Kong J, Chia L, Goh N, Chia T, Brouillard R (2003). Analysis and biological activities of anthocyanins. Phytochemistry, 64: 923-933.

Kraemer-Schafhalter A, Fuchs H, Pfannhauser W (1998). Solid-phase extraction (SPE) - a comparison of 16 materials for the purification of anthocyanins from Aronia melanocarpa var Nero, Journal of the Science of Food and Agriculture, 78(3)435-440.

Rein M (2005). Copigmentation reactions and color stability of berry anthocyanins. University of Helsinki, Department of Applied Chemistry and Microbiology, Food Chemistry Division

Rugină D, SconÅ£a Z, Leopold L, Pintea A, Bunea A, Socaciu C (2012). Antioxidant Activities of Chokeberry Extracts and the Cytotoxic Action of Their Anthocyanin Fraction on HeLa Human Cervical Tumor Cellsâ€, Journal of Medicinal Food, 15, TBD, 15(8): 700-706

Markham KR, Gould KS, Winefield CS, Mitchell KA, Bloor SJ, Boase MR (2000). Anthocyanic vacuolar inclusions - their nature and significance in flower colouration. Phytochemistry, 55: 327-336.

Mazza G, Miniati E (1993). Anthocyanins in fruits, vegetables and grains. CRC Press, Ino; Boca Raton, FL

Pavlovic DM, Babic S, et al (2010). Development and optimization of the SPE procedure for determination of pharmaceuticals in water samples by HPLC-diode array detection. Journal of Separation Science, 33: 258-267.

Pazmino-Duran EA, Giusti MM, Wrolstad RE, Gloria MBA (2001). Anthocyanins from banana bracts (Musa paradisiaca) as potential food colorants. Food Chem, 73:327-332.

Pecket RC, Small CJ (1980). Occurrence, location and development of anthocyanoplasts. Phytochemistry, 19:2571–2576.

Rodriguez-Saona LE, Wrolstad RE (2001). Extraction, isolation, and purification of anthocyanins. Current Protocols in Food Analytical Chemistry. Hoboken, NJ, John Wiley & Sons, Inc.: F1.1.1-F1.1.11

Segura-Carretero A, Puertas-Mejía MA, Cortacero-Ramírez S, Beltrán R, Alonso-Villaverde C, Joven J, Dinelli G, Fernández-Gutiérrez A (2008). Selective extraction, separation, and identification of anthocyanins from Hibiscus sabdariffa L. using solid phase extraction-capillary electrophoresis-mass spectrometry (time-of-flight /ion trap). Electrophoresis, 29(13):2852-61.

Socaciu C (2007). Food colorants: chemical and functional properties. Boca Raton: Taylor & Francis. 616-617

Simmons ST (2011). Commercial Applicability of an Innovative Anthocyanin Purification Technique, Utilizing Mixed-Mode Solid-Phase Extraction, The Ohio State University

Waterhouse AL (2002). Wine Phenolics. Ann. N.Y. Acad. Sci., 957, 21-36.

Xiaoke H, Zhimin X (2011). Chapter 15. Isolation Characterization of Bioactive Compounds in Fruits and Cereals Fruit and Cereal Bioactives Edited by Özlem Tokuşoğlu and Clifford Hall III CRC Press ,319–336 Print ISBN: 978-1-4398-0665-4

DOI: http://dx.doi.org/10.15835/buasvmcn-fst:11986


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