Stability Comparison of Free and Encapsulated Lactobacilus casei ATCC 393 in Yoghurt for Long Time Storage
Abstract
An innovative method of L. casei ATCC 393 encapsulation has been reported in the present study using pectin combined with alginate. The aim of this study was to investigate the effect of encapsulation on the survival of L. casei ATCC 393 in yoghurt during long time storage, free or encapsulated in alginate and alginate pectin microspheres, and influence over yoghurt properties, particularly acidification. Over 35 days of storage in yoghurt, the encapsulated probiotic cells proved a higher viability compared with free probiotic cells. An even higher viability and stability was observed for the samples where pectin was used. Pectin acts as prebiotic during encapsulation of L. casei ATCC 393.
References
Champagne, CP, Gardner NJ Roy D (2005). Challenges in the Addition of Probiotic Cultures to Foods. Critical Reviews in Food Science and Nutrition 45:61-84.
Dave, RI Shah NP (1997). Viability of yoghurt and probiotic bacteria in yoghurts made from commercial starter cultures. Int Dairy J 7:31-41.
De Vuyst, L Degeest B (1999). Heteropolysaccharides from lactic acid bacteria. FEMS Microbiol Rev 23:153-177.
Delzenne, N, C D, A N, M L HS T (2002). Inulin and oligofructose modulate lipid metabolism in animals: review of biochemical events and future prospects. British Journal of Nutrition 87:S255–S259.
Gebara, C, Chaves KS, Ribeiro MCE, Souza FN, Grosso CRF Gigante ML (2013). Viability of Lactobacillus acidophilus La5 in pectin-whey protein microparticles during exposure to simulated gastrointestinal conditions. Food Res Int 51:872-878.
Gilliland, SE, Reilly SS, Kim GB Kim HS (2002). Viability During Storage of Selected Probiotic Lactobacilli and Bifidobacteria in a Yogurt-like Product. Journal of Food Science 67:3091-3095.
Gilliland, SE Speck ML (1977). Instability of Lactobacillus acidophilus in Yogurt. J Dairy Sci 60:1394-1398.
Hansen, LT, Allan-Wojtas PM, Jin YL Paulson AT (2002). Survival of Ca-alginate microencapsulated Bifidobacterium spp. in milk and simulated gastrointestinal conditions. Food Microbiol 19:35-45.
Jackson, LS Lee K (1991). Microencapsulation in food industry. Lebensmittel Wissenschaft und Technologie 24:289-297.
Kailasapathy, K (2002). Microencapsulation of probiotic bacteria: technology and potential applications. Current issues in intestianl microbiology 3:39-48.
Kailasapathy, K (2006). Survival of free and encapsulated probiotic bacteria and their effect on the sensory properties of yoghurt. LWT - Food Science and Technology 39:1221-1227.
Kailasapathy, K J. C (2000). Survival and therapeutic potential of probiotic organisms with reference to Lactobacillus acidophilus and Bifidobacterium spp. Immunol Cell Biol 78:80-88.
Laparra, JM Sanz Y (2010). Interactions of gut microbiota with functional food components and nutraceuticals. Pharmacological Research 61:219-225.
Lourens-Hattingh, A Viljoen BC (2001). Yogurt as probiotic carrier food. Int Dairy J 11:1-17.
Nazzaro, F, Fratianni F, Nicolaus B, Poli A Orlando P (2012). The prebiotic source influences the growth, biochemical features and survival under simulated gastrointestinal conditions of the probiotic Lactobacillus acidophilus. Anaerobe 18:280-285.
Patel, PJ, Singh SK, Panaich S Cardozo L (2013). The aging gut and the role of prebiotics, probiotics, and synbiotics: A review. Journal of Clinical Gerontology and Geriatrics.
Saad, N, Delattre C, Urdaci M, Schmitter JM Bressollier P (2013). An overview of the last advances in probiotic and prebiotic field. LWT - Food Science and Technology 50:1-16.
Saulnier, DMA, Spinler JK, Gibson GR Versalovic J (2009). Mechanisms of probiosis and prebiosis: considerations for enhanced functional foods. Curr Opin Biotechnol 20:135-141.
Sultana, K, Godward G, Reynolds N, Arumugaswamy R, Peiris P Kailasapathy K (2000). Encapsulation of probiotic bacteria with alginate-starch and evaluation of survival in simulated gastrointestinal conditions and in yoghurt. Int J Food Microbiol 62:47-55.
Tannock, GW, Munro K, Harmsen HJM, Welling GW, Smart J Gopal PK (2000). Analysis of the Fecal Microflora of Human Subjects Consuming a Probiotic Product Containing Lactobacillus rhamnosus DR20. Appl Environ Microbiol 66:2578–2588.
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