Contributions to the Knowledge of Microbiological Quality of Pasteurized Melange Produced in Romania

Andreea Catalina NISTOR, Lucia Iuliana NISTOR, Claudia Gabriela DUMINICA, Marius Giorgi USTUROI


Today's consumers show an affinity for quality products produced under production conditions that strictly observes the principles of food safety. Bacterial contamination of eggs and egg products can thus be a source of disease for the human consumer with the necessary effects on the sector of activity.

The term "egg products" defines the components of the egg (separate or as a mixture-melange) released from their shell and submitted to a heat treatment, they are forming foods rich in protein, vitamins and trace elements of high quality .

The purpose of this study was to establishing and monitoring the microbiological indicators of melange produced in our country.

The study was carried out on the pasteurized egg mix, packaged "bag-in-box" (5 units package), and stored for a period of 25 days at the temperature recommended by the manufacturer (0 ... + 4 °C). The actual analysis is to determine NTGMA, Enterobacter spp., Bacillus spp., Pseudomonas spp., and Salmonella spp.

Regarding developments of NTGMA in pasteurized melange stored for 25 days, our data showed an increase of 75.22% compared to the situation found on fresh product (3.656 log cfu/ml vs 6.406 log cfu/ml). The situation was similar for Enterobacter spp. (119.64% increase), Bacillus spp. (76.27% increase) and Pseudomonas spp. (58.72% increase). Salmonella spp. was not identified in the product tested by us.

The heat treatment of pasteurized liquid melange does not destroy completely the initial microbial load, therefore certain precautions are needed to store these foods.


melange, microbiological indicators, quality

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Aragon-Alegro Lina Casale, Souza Kátia Leani de Oliveira, Costa Sobrinho Paulo de Souza, Landgraf Mariza, Destro Maria Teresa (2005). Avaliação da qualidade microbiológica de ovo integral pasteurizado produzido com e sem a etapa de lavagem no processamento. Ciênc. Tecnol. Aliment., Campinas, 25(3): 618-622.

Board R. G., Fuller R. (1994). Microbiology of the avian egg, Ed. Chapman& Hall, p. 63–92, London.

Chmielewski, R. A., Beck, J. R., Juneja, V. K., Swayne, D. E. (2013). Inactivation of low pathogenicity notifiable avian influenza virus and lentogenic Newcastle disease virus following pasteurization in liquid egg products. LWT - Food Science and Technology, vol. 52, no. 1, p. 27-30.

Cwiková Olga, Nedomová Šárka(2014). Microbiological quality of egg liquid products. Potravinarstvo Scientific Journal for Food Industry, vol. 8, 2014, no. 1, p. 114-118.

Garcia-Gonzalez L., Geeraerd A.H., Spilimbergo S., Elst K,. Van Ginneken L., Debevere J., Van Impe J.F., Devlieghere F. (2007). High pressure carbon dioxide inactivation of microorganisms in foods: the past, the present and the future. International Journal of Food Microbiology, nr. 117, pp. 1–28.

Labbi R. G. and Garcia S. (2001). Guide to foodborne pathogens. Ed. Wiley-Interscience, p. 1–22, New York.

Nau Françoise, Guérin-Dubiard Catherine, Baron Florance, Thapon J. L.(2010). Science et technologie de lʼœuf, Vol. 2, Ed. Lavoisier, p. 321-347, Paris.

Németh C., Mráz B., Friedrich L., Suhajda A., Janzsó B., Balla C.(2011). Microbiological measurements for the development of a new preservation procedure for liquid egg. Czech J. Food Sci., vol. 29, p. 569-574.

Patrignani F., Vannini L., Kamdem S. L. S., Hernando I., Marco-Molés R., Guerzoni M. E., Lanciotti R. (2013). High pressure homogenization vs heat treatment: Safety and functional properties of liquid whole egg. Food Microbiology, vol. 36, no. 1, p. 63-69.

Protais Jocelyne, Boscher Evelyne, Quéguiner Stéphane, Chidaine B., Fravalo P.(2007) Identification du genre Pseudomonas dans la matrice « oeuf entier liquide », Septièmes Journées de la Recherche Avicole, p 524-527.

Protais Jocelyne, Gerault P., Queguiner Stéphane, Boscher Evelyne, Chidaine B., Emel Gwennola, Rivoal Katell, Salvat G., Pages J., Thuault Dominique, Huchet Véronique, Coignard Muriel, Bourion Fabrice, Federighi M., Jugiau Florence, Thouvenot D., Efstathiou T., Lorthioir P.(2006) Identification et comportement des bactéries d'altération dans la matrice oeuf entier liquide, Sciences et Techniques Avicoles, no. 57, p 4-13.

Rossi M., Casiraghi E., Primavesi L., Pompei C., Hidalgo A. (2010). Functional properties of pasteurised liquid whole egg products as affected by the hygienic quality of the raw eggs. LWT - Food Science and Technology, vol. 43, no. 3, p. 436-441.

Simeonovová, J. 2013. Technologie drůbeže, vajec a minoritních živoÄiÅ¡ných produktů.2nd ed. Brno: Mendelova univerzita v BrnÄ›, 241 p. ISBN 978-80-7375-891-2.

SR EN ISO 13720:2011 - Meat and meat products -- Enumeration of presumptive Pseudomonas spp.

SR EN ISO 6579:2007/A1 - Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Salmonella spp.

SR ISO 21528-2:2007 - Microbiology of food and animal feeding stuffs -- Horizontal methods for the detection and enumeration of Enterobacteriaceae -- Part 2: Colony-count method.

SR EN ISO 7932:2005 Microbiology Of Food And Animal Feeding Stuffs - Horizontal Method For The Enumeration Of Presumptive Bacillus Cereus by means of the colony - count technique at 30°C.

Wong, P. Y. and Kitts, D. (2003). Physicochemical and functional properties of shell eggs following electron beam irradiation. Journal of the Science of Food and Agriculture, no 83, p. 44–52.



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