Bioactive Labels for Fresh Fruits and Vegetables

  • Nasui Liana Usamv Cluj
  • Dan Vodnar University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca
  • Socaciu Carmen Usamv cluj
Keywords: HPLC, bioactive lables, green tea, safety.

Abstract

Pesticide residues and microbial load on the surface of fresh fruits and vegetables becomes a major concern due to the safety and quality of these products for consumer.In order to minimize these risk factors (pesticide residues and microbial load), were achieved labels for fruits and vegetables that are consumed with shell which disintegrates under the influence of water jet and thus reduce the amount of these contaminants. Were elaborated labels based on polymer (chitosan) at a concentration of 2%, which incorporate bioactive compounds from green tea with potential decontaminant of the peel of this products. Green tea extract was obtained by infusing 1 g of dried green tea in 100 ml water at 80° C for 10 minutes. The extract was filtered and then mixed with 2 g chitosan acidified with 0.7% glacial acetic acid and dilute to the mark with distilled water. Were identified bioactive compounds from green tea, using UV-VIS and HPLC. Then were elaborated the labels. These tags were used on pepper, tomato, apple and  nectarine. Were quantified the microbial load and the pesticide residues on their surface unwashed, washed only with water and were monitored the influence of labels on these factors. Identified pesticides were mefenoxan and thiamethoxam, which were quantified by HPLC. In what it concerns the influence, were founded the absence of germs at pepper and a significant decrease at the other. In terms of  the potential of reducing pesticide, the experimental results have indicated that the label can prove its effectiveness.

 

Author Biography

Dan Vodnar, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca
Chemistry and Biochemistry

References

Alschuler L (1998). Green Tea: Healing tonic. Am J Natur Med, 5, 28-31.

Apostu S.( 2009). Microbiologia produselor alimentare, Vol II, Ed. Risoprint, Cluj-Napoca, p. 243-252.

Apostu S., Ancuța M. Rotar (2009). Microbiologia produselor alimentare. Lucrări practice, Vol.III, Ed. Risoprint, Cluj-Napoca, 189-191.

Devgan K. (2012). Why fruit, vegetable peels are good for you. Hindustan Times, New Delhi 1-2.

Dong H., Cheng L., Tan J., Zheng K., Jiang Y. (2004). Effects of chitosan coating on quality and shelf life of peeled litchi fruit. J. Of Food Engineering, 64(3), China, 355-358.

Graham N. H.., (1992) Green tea composition, consumption, and polyphenol chemistry. Preventive medicine 21(3), USA, 334-350.

Hamilton-Miller J. M. T. (1995). Antimicrobial properties of tea (Camellia sinesis L.). Antimicrobial Agents and Chemotherapy, 39(11), United Kingtom, 2375-2377.

Keikothaile B.M., P. Spanoghe, W.Steurbaut (2010). Pesticide Residues in fruits and vegetables. Food and Chemical Toxicology 48(1) , Belgium, 244-249.

Kim K-Y., Oh J-Y, Chung O-J, Lee J-S, Kim O-K (2009). Chemical composition of green tea according to processing methods and extraction conditions. Food Sci. Biotechnol. 18(5), Korea, 1212-1217.

Krol, W.J., Arsenault T.L., Pyplypiw, H.M., Mattina M.J.I. (2000). Reduction of pesticide residues on produce by rinsing. J.Agric. Food Chem. 48, New Haven, 4666-4670.

Kumar A., Kumar A., Thakur P., Patil S., Payal C., Kumar A. (2012). Antibacterial activity of green tea (Camellia sinesis) extracts against various bacteria isolated from environmental sources. Recent Research in Science and Technology 4(1), India, 9-23.

Liu F. X., Guan L. Y., Yang Z. D., Li Z., Yao D. K. (2001). Antibacterial action of chitosan and carboxymethylated chitosan. J. Of Applied Polymer Science. 79, 1324-1335.

Liu H., Du Y., Wang X., Sun L., (2004). Chitosan kills bacteria through cell membrane damage. Int. J. Of Food Microbiology 95(2), China, 147-155.

Lu C. Li, Wang I. C., Sye F. W. (2010). Applications of chitosan beads and porous crab shell powder for the removal of 17 organochlorine pesticides (OCPs) in water solution. Carbohydrate Polymers 83(4), Taiwan, 1984-1986.

Reto M., Figueira E. M., Filipe M. H., Almeida M. M. C. ( 2007), Chemical Composition of Green Tea (Camellia sinensis) Infusions Commercialized in Portugal. Plant Foods for Human Nutrition 62(4), Portugal 139-144.

Roller S., N. Covill (1999). The antifungal properties of chitosan in laboratory media and apple juice. International 47(1-2), London, UK, 67-77.

Rugină O.Dumitrița (2009). Caracterizarea unor extracte vegetale bogate în flavan-3-oli cu potențial antioxidant și apoptotic. Rezumatul tezei de doctorat, Cluj-Napoca, 11.

Vodnar C. D., Socaciu Carmen (2012). Green tea increases the survival yield of Bifidobacteria in simulated gastrointestinal environment and during refrigerated conditions. Chemistry Central Journal 6(61), Cluj-Napoca, 2-3.

Zaferi T. N. (2006). Green tea and its polyphenolic catechins: Medicinal uses in cancer and noncancer applicatios. Life Science 78, USA, 2073-2080.

Zander A. and Bunning M. (2010). Guide to Washing Fresh Produce. Food and Nutrition Series/ Food Safety 9.380, Colorado, 1-2.

Published
2014-03-31