DETERMINATION OF FLAVONOIDS FROM VIOLA ARVENSIS AND V. DECLINATA (VIOLACEAE)

Anca TOIU, Ilioara ONIGA, Laurian VLASE

Abstract


Flavonoids are plant-derived dietary polyphenols with major role as anti-oxidant and oxidative stress preventive molecules. Viola species (Violaceae) are medicinal plants used in traditional medicine for their anti-inflammatory, diuretic and expectorant properties. The aim of the study was to analyze the flavonoids from aerial parts of Viola arvensis Murray and V. declinata Waldst. et Kit. (Violaceae). The identification of compounds was made by high-performance liquid chromatography (HPLC) coupled with mass spectrometry (ESI-MS), whereas the quantitative determinations by spectrophotometric methods. Ethanolic extracts of the vegetal products were studied before and after acid hydrolysis. Isoquercitrin and rutoside were identified in both species before hydrolysis and quercetol and kaempferol after hydrolysis.

Keywords


Viola arvensis, V. declinata, flavonoids, rutoside, LC-MS

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