COMPARATIVE STUDY OF THE HOP BITTER ACIDS ANALYSIS METHODS

Maria Tofana

Abstract


A single laboratory validation study (SLV) was conducted for an conductometric method for the determination of total hop acids in pellets from different variety. Extraction variants were developed in accordance with Analytica EBC 7.4 which is identical with Romanian Standard SR 13842:2003, where the extraction solvent is toluene (Extraction System A) and with accordance with Analytica EBC 7.5 and 7.6 where the extraction solvent is formed by diethyl ether, methanol and hydrochloric acids (Extraction System B). The procedure consisted of parallel determination of hop acids in variety with low and high content of alpha acids. For one variety the determination where conducted also in HPLC, using a reverse phase C18 column.
The determination where performed in repeatability and reproducibility conditions. The recovery determined was about 102 %.

Keywords


hop; bitter acids; HPLC; conductometric value

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