GENETIC MAPPING OF WHEAT 2BL CHROMOSOME USING SSR MARKERS
Abstract
The formation of albino plants is among the major hurdles against using the anther culture and isolated microspore culture to produce homozygous plants for new valuable cultivars. Regeneration of albino plants is a phenomenon associated with cereal in vitro culture. The ability to form a high percentage of green plant has been found to show a considerable genetic component. Researches with anther culture response from reciprocal crosses between high and low responsive lines established that the genes for embryo and green plant formation in wheat and barley are mainly chromosomally inherited. Molecular markers, were used to locate the genes responsible for green plant formation. There were identified three QTLs on wheat chromosomes 2AL (QGpp.kvl-2A), 2BL (QGpp.kvl-2B.1) and 5BL (QGpp.kvl-5B) that affects the capacity of regeneration of green plants from anther culture in a double haploids population of wheat (Ciano x Walter). Subsequently, two double haploid lines, CW204 and CW219 from the original mapping population Ciano x Walter were used as parents to establish a mapping population segregating for the QTL region on chromosome 2BL affecting green plant formation to confirm location of the QTL on 2BL. The approximately 30 cM chromosomal region on the long arm of 2BL, associated with the green plant formation, was mapped before, with a number of microsatellite markers. In this study there are mapped some other microsatellite markers on the long arm of the chromosome 2B, to generate a more dense map of the region of interest, in order to locate more exactly the gene responsible for the green plant formation in wheat.Authors who publish with this journal agree to the following terms:
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