The Influence of the Gelling Agent and Explant Type upon the In Vitro Multiplication of Pinguicula vulgaris

Doina CLAPA, Alexandru FIRA, Ioan PACURAR, Anca SOTROPA


Our paper presents results regarding in vitro multiplication of species Pinguicula vulgaris using non-conventional gelling agents and, as explants, whole rosettes as well as rosette fragments. As nutritive medium, Murashige & Skoog 1962 (MS) with 0.5 mg/l 6-Benzylaminopurine (BAP) was used, and the gelling agents were Plant Agar at 6 g/l, Guar gum at 10 and 20 g/l and starch at 70 g/l. The highest number of plantlets/vessel (619) was obtained by using, as explants, rosette fragments and, as gelling agent, 20 g/l Guar gum and the lowest multiplication rate was obtained by using whole rosettes as explants, on the same medium. The explants consisting of rosette fragments generated, on the MS medium gelled with either Guar gum or starch a far larger number of plantlets as compared to the media gelled with agar and the explants consisting of rosette fragments generated a very large number of plantlets as compared to the explants consisting of whole rosettes.

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