The in vitro Establishment of Walnut (J. regia L.) by Using two Alternative Gelling Agents first Results

Rodica GOTEA, Mohammad Ali SHEIKH BEIG GOHARIZZI, Ionuț GOTEA, Mohammad ZIAEE, Mina FARSI, Radu E. SESTRAS, Kourosh VAHDATI

Abstract


The in vitro establishment of three Romanian walnut cultivars (‘Mihaela’, ‘Muscelean’ and‘Jupânești’) from microcuttings was studied by using two alternative gelling agents (cassava flour andtapioca) and DKW basal medium. The agar concentration was established at 8 g/L and theconcentrations for the other two gelling agents where established at 150 g/L for cassava flour and at 15g/L for tapioca. The best growth was recorded for ‘Jupânești’ cultivar on the DKW basal medium withBAP (1 mg/L) and solidified with tapioca (15 g/L).

Keywords


Manihot esculenta, agar, micropropagation

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DOI: http://dx.doi.org/10.15835/buasvmcn-hort:8554


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