Molecular Characterisation of Some Carnivorous Plants Using RAPD Markers

  • Rodica POP USAMVCN
  • Doru PAMFIL USAMVCN
  • Elena RANBA UBBCN
  • Monica HARTA USAMVCN
Keywords: carnivorous plants, DNA, RAPD, genetic variability

Abstract

In the present study we use 23 RAPD decamers to characterise 12 carnivorous plants species included in four different genera with the main goal to analyze the intra and inter relationships at DNA molecular level. RAPD analysis was a valuable tool for molecular characterisation of the carnivorous plants. Primers of OPB, OPA, OPE, AB, OPAL series generated the many bands (12-19) in the range of 200-1500 bp, with high level of polymorphism (77.7- 100%). The UPGMA dendrogram built  using TreeView software, based on the genetic relationships between carnivorous plants, confirmed the high level of variability existing among Drosera species although between some analyzed species was not observed evident differences on the leaf morphology, prey capture system and plant height. RAPD assays showed that Sarracenia purpurea and Nepenthes northiana were clustered more closely than Dionea muscipula.

Published
2013-11-21