The Effect of Selenium and Zinc Supplementation on Growth and Reproduction of the Mulberry Silkworm (Bombyx mori L.)

Mihai Bențea, Liviu Alexandru Mărghitaș, Aurel Șara, Daniel Dezmirean, Erol Gabor, Bogdan Vlaic, Călina Creța

Abstract


This research aimed to evaluate the influence of Se and Zinc, administered as food supplements for silkworm
(Bombyx mori L.) on the larvae weight, the weight of the serigene gland and on some reproduction indices (prolificacy and fecundity). The biologic material was represented by the Baneasa White variety. The research has been carried out on 9 groups of silkworms one control group (G1) and 8 experimental groups (4 groups that received zinc and 4 groups that received Selenium), consisting of 50 larvae/group. Zinc was administered to 4 experimental groups, in doses of 17, 34, 68 and 136 mg kg
-1
larvae (G2-G5). Selenium was administered to the other 4 experimental groups, in doses of 4, 8, 16 ad 32 mg kg
-1
body weight (G6-G9). The minerals were mixed with water to form a solution and the solution was pulverized on the mulberry leafs. The larvae were weighted in the 1
st
, 4
th
and 7
th
day from the 5
age. The serigene glands were collected in the 7
th
day of the same age and the reproduction parameters were analyzed a few days after the eggs were laid down by the female butterflies. The use of Zinc determined a very significant increase of the larvae weight, to group G5 followed by groups G4, G3 and G2 compared to the control group. The use of Zinc positively influenced the mass of the serigene glands, the number of eggs laid, and the number of fertilized eggs laid by the female butterflies. The use of organic Selenium determined an increased larvae weight, the differences being very significant compared to the control group. Selenium influenced the weight of the serigene gland, the number of eggs laid and the number of fertilized eggs.

Keywords


silkworm, Bombyx mori L., minerals, mulberry leaf, alimentation

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