Changes Evaluation of Reserve Substances and Degradation Enzymes after Exposure of Tomato Plants (Lycopersicon esculentum Mill.) to Alpha - Cypermethrin, Chlorpyriphos and Pyrimicarb

Karim Chahid, Amin Laglaoui, Said Zantar, Abdeslam Ennabili

Abstract


Tomato (Lycopersicon esculentum Mill.) is one of the most important vegetables, whose production and consumption increased quite rapidly. The impact of three xenobiotics such as alpha-cypermethrin, chlorpyriphos and pyrimicarb on reserve substances (proteins, starch and lipids) and degradation enzymes (protease and alpha-amylase) was investigated. The effect of the insecticides was observed by using four dilutions of the normal concentration used in agriculture (100%, 75%, 50% and 25%) for germinating seeds, and only the recommended concentration in agriculture for growing plants. The results suggest that the tested insecticides induced an accumulation of proteins in both treated seeds, and treated plants leaves and roots. Moreover, the protease activity was reduced in treated seeds and plants. Also a great accumulation of starch in presence of the insecticides was registered in treated seeds, and leafs and roots of treated plants, whereas this accumulation is accompanied with an inhibition of alpha-amylase activity. Concerning lipids, a significant increase was observed in treated samples compared to the control ones.


Keywords


insecticides, tomato, Lycopersicon esculentum Mill., reserve substances, degradation enzymes

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