The Arabidopsis thaliana Glycine-rich RNA Binding Proteins atGRP7 and atGRP2 Are Involved in Early Development

Oana Ciuzan, Michael Ladomery, Ian Wilson, John Hancock, Doru Pamfil

Abstract


The glycine-rich RNA-binding proteins of Arabidopsis thaliana are of growing interest in the context of understanding how plants respond to biotic and abiotic stresses. In this study we focus on the phenotypic analysis of Arabidopsis knock-out mutants of genes that encode the glycine-rich proteins atGRP2 and atGRP7, comparing them to knock-out mutants of ABI3, ABI4 and ABI5, genes linked to cell signaling through the plant hormone abscisic acid (ABA). The results show that the abscisic acid insensitive (ABI) mutants developed faster than wild-type plants. In contrast the glycine-rich protein knock-out mutants (grp7-1 and grp2) did not grow as well, suggesting an involvement of these genes in key early developmental processes. There was a significant difference in the phenotype of the knock-out mutants grp2 and grp7-1, suggesting that atGRP7 and atGRP2 have overlapping yet distinct roles in development.


Keywords


Arabidopsis thaliana, glycine-rich proteins (GRP), abscisic acid (ABA), abscisic acid insensitive mutants (ABI).

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