Identification and Quantification of Fatty Acids in Cyanobacteria Cells

Sanda OPRIS, Cosmin SICORA, Teodor RUSU, Mirela MICLEAN, Alina CREHUL

Abstract


Cyanobacteria biomass lipid extraction was performed by the method of Bligh and Dyer [5] in chloroform/methanol mixture and methanol/water mixture. The mixture separation in three specific phases made ​​possible the lipid extraction in chloroform (lower phase). Quantitative determination of fatty acids was performed by gas-liquid chromatography of the fatty acid methyl esters using an Agilent 7890 N gas chromatograph. Analyses were performed in the Environmental Analysis Laboratory of the Research Institute for Analytical Instrumentation, ICIA Cluj by the means of specialist Mrs. Miclean M. Data processing was performed using the OriginPro 8 program.


Keywords


cyanobacteria, lipid extraction, chloroform, methanol, chromatography, fatty acids.

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