Impact of Isoflurane and Sevoflurane Anesthesia on Kidney Structure and Function in Rats

Flavia RUXANDA, Viorel MICLAUS, Vasile RUS, Adrian Florin GAL, Liviu OANA


Various researchers studied the nephrotoxicity of inhalational anesthetics, with contradictory results. We aimed to observe the impact of the repeated isoflurane or sevoflurane anesthesia on kidney structure and function. 40 female rats were used in this study. Animals were divided in 8 groups. Two groups served as control: IM (isoflurane) and SM (sevoflurane), exposed to oxygen alone (1l/min) 3 times, for 2 hours, every other day. Three groups were anesthetized with isoflurane (I1, I2, I3) and the other three (S1, S2, S3) with sevoflurane. The anesthetics were administered three times, for two hours long each, every other day. Rats were sacrificed through cervical dislocation as follows: groups IM, SM, I1, S1 imediately after the last anesthesia, groups I2, S2 - 6 hours post-anesthesia and groups I3, S3 - 24 hours post-anesthesia. Kidney samples were harvested, fixed in 10% buffered formaline, embedded in paraffin, sectioned (5 µm thickness) and stained with Goldner’s trichrome metod. After isoflurane anesthesia, the kidney displays a moderate congestion in the corticomedullary area, which maintains throughout the experimental period. The only changes in animals anesthetized with sevoflurane also appear on the vascular component. The congestion maintains throughout the experimental period in the renal cortex, as well as the corticomedullary area. No other aspects that suggest functional impairment are observed, in any of the experimental groups. Inhalational anesthesia with isoflurane and sevoflurane produces a moderate congestion, without majorly impairing the kidney function.


congestion; isoflurane; kidney; sevoflurane

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