Oxidative Stress Evaluation in Rats Anesthetized with Isoflurane or Sevoflurane

  • Flavia Ruxanda University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
  • Viorel Miclaus University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
  • Vasile Rus University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
  • Adrian Florin Gal University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
  • Liviu Oana University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
Keywords: glutation peroxidase, isoflurane, sevoflurane, superoxid dismutase

Abstract

Oxidative stress implies an imbalance between antioxidant production and reactive oxygen species generation. In this experimental study, we aimed to assess the effects of two inhalational anesthetics (isoflurane and sevoflurane) on the oxidative stress enzymes, namely: superoxid dismutase and glutathione peroxidase. Our protocol included 40 Wistar female rats, divided into 8 groups (5 animals per group): IM, I1, I2, I3, SM, S1, S2 and S3. The groups were subdued to: oxygen alone (control groups IM and SM), isoflurane (I1, I2, I3) and sevoflurane anesthesia (S1, S2, S3), 3 times, for 2 hours long, every other day. The supplied oxygen was 1l O2/min and the concentration of isoflurane and sevoflurane was 1 minimum alveolar concentration (1 MAC). We harvested blood samples from each group at different moments: IM, SM, I1, S1 - immediately, I2, S2 - 6 hours and I3, S3 - 24 hours after the treatment. We assessed superoxid dismutase and glutathion peroxidase in the red blood cells. Both anesthetics drecreased the enzyme activities: isoflurane in groups I1 and I3, whereas sevoflurane in all experimental groups (S1, S2, S3).

Author Biographies

Flavia Ruxanda, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
Department of Anesthesiology and Surgical Propedeutics - PhD student
Viorel Miclaus, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

Department of Cell Biology, Histology and Embriology - Professor, Ph.D

Vasile Rus, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
Department of Cell Biology, Histology and Embriolog -Lecturer, Ph.D
Adrian Florin Gal, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

Department of Pathological Anatomy - Lecturer, Ph.D

Liviu Oana, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

Department of Anesthesiology and Surgical Propedeutics - Professor, Ph.D

Published
2015-05-29