Oxidative Stress Evaluation in Rats Anesthetized with Isoflurane or Sevoflurane

Flavia Ruxanda, Viorel Miclaus, Vasile Rus, Adrian Florin Gal, Liviu Oana


Oxidative stress implies an imbalance between antioxidant production and reactive oxygen species generation. In this experimental study, we aimed to assess the effects of two inhalational anesthetics (isoflurane and sevoflurane) on the oxidative stress enzymes, namely: superoxid dismutase and glutathione peroxidase. Our protocol included 40 Wistar female rats, divided into 8 groups (5 animals per group): IM, I1, I2, I3, SM, S1, S2 and S3. The groups were subdued to: oxygen alone (control groups IM and SM), isoflurane (I1, I2, I3) and sevoflurane anesthesia (S1, S2, S3), 3 times, for 2 hours long, every other day. The supplied oxygen was 1l O2/min and the concentration of isoflurane and sevoflurane was 1 minimum alveolar concentration (1 MAC). We harvested blood samples from each group at different moments: IM, SM, I1, S1 - immediately, I2, S2 - 6 hours and I3, S3 - 24 hours after the treatment. We assessed superoxid dismutase and glutathion peroxidase in the red blood cells. Both anesthetics drecreased the enzyme activities: isoflurane in groups I1 and I3, whereas sevoflurane in all experimental groups (S1, S2, S3).


glutation peroxidase, isoflurane, sevoflurane, superoxid dismutase

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DOI: http://dx.doi.org/10.15835/buasvmcn-vm:10389

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