Biochemical and Antioxidant effect of Doxorubicin hydrochloride and Propolis on N-methyl-N-nitrosourea (MNU) Induced Adenocarcinoma in Rats
Abstract
The present work was performed to study the anticancer effect of doxorubicin HCL and propolis and their combination in the treatment of mammary cancer induced by N-methyl-N-nitrosourea(MNU) beside the ameliorating effect of propolis against side-effects of doxorubicin HCL using the clinicobiochemical and histopathological changes.One hundred and twenty five female white albino rats (two months old and 100 gm body weight) were divided into five equal groups .Starting of experiment by induction of carcinogen N-methyl-N-nitrosourea (MNU) into gps.(2-5) while rats kept as control in gp.(1). After incidence of mammary tumor in gps. (2-5) begin the treatment which continues for 70 days. Rats were given carcinogen only, doxorubicin HCL, propolis and combination of therapeutic dose from doxorubicin HCL and propolis in gps.(2,3,4 and 5),respectively. Blood samples were collected after 120 days from carcinogen induction and (30 and 70 days) post-treatment. Elevated serum CA 15.3 level in rat gp.(2) injected with carcinogen MNU after 120 days. Serum CA 15.3 showed a significant decrease in gps.(3 and 5) especially 70 days post-treatment.Results of Gp.(3) showed increasing serum ALT, AST and ALP activities and creatinine and urea level and hepatic (MDA) assay in addition to decrease serum protein and albumin and hepatic (CAT) and (SOD) activities 30 and 70 days post-treatment. These changes were ameliorated in gp.(5). Gp.(4) showed improvement of the previous parameters. Histopathological finding confirm these investigations. So it is concluded that doxorubicin HCL caused hepatic and renal injuries and had cumulative side effects (increasing side effects by increasing intake duration). The propolis did not induce side effects and possessed some anticancer activity but lower than doxorubicin HCL. Combination of doxorubicin HCL with propolis decreased side effects of doxorubicin HCL and increased anticancer activity of the propolis.
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