Measurement of Mean Arterial Pressure by Carotid Artery Cannulation in Isoflurane Anesthetized Wistar Rats

  • Sidonia Alina BOGDAN Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine http://orcid.org/0000-0002-4060-2992
  • Vlad LUCA Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine
  • Cosmin PESTEAN Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine
  • Ciprian OBER Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine
  • Lucia BEL Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine
  • Andrei Razvan CODEA Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine
  • Ileana BOGDAN Departent of Technical and Soils Sciences, University of Agricultural Sciences and Veterinary Medicine
  • Radu MANDEAL Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine
  • Liviu OANA Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine
Keywords: carotid cannulation, mean arterial pleasure, rats

Abstract

Experimental studies on small lab animals, particularly rats are a widely-used model (Ordodi et. all 2004). Blood pressure measurement is one of the fastest and most informative means of assessing cardiovascular function and provides an accurate indication of drug effects, surgical events and hemodynamic trends (Tranquilli et. all, 2007). A close monitoring of the animals during the experiment is important to detect early imbalances and prevent any changes that could compromise the results.

Blood pressure measurement is one of the basic procedures in biomedical research and it can be done by non-invasive or invasive technics. The gold standard procedures with the most accuracy are the invasive technics. This includes radial, femoral, brachial or carotid cannulation (Parasuraman et. all 2012). Carotid artery catheterization is a common procedure that allows precise monitoring of the blood pressure but surgery is required to perform them (Plehm et. all 2006).

Experimental surgery on small lab animals, especially rats, it’s a widely used model. In order to perform surgery, anaesthesia must be used and it usually causes a slowdown of the respiration that induces metabolic imbalances. It could cause cardiovascular collapse and death if left unbalanced. A close monitoring of the respiration, ECG, blood pressure is necessary during the experiment (Ordodi et. all 2004).

Because it may not be always available or the prohibitive cost of the special equipment used to perform these tasks, we adapted a simple device and method to record the mean arterial pressure (MAP) in rats by carotid catheterisation and the results obtained were sustainable.

Author Biographies

Sidonia Alina BOGDAN, Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine
Phd Student
Vlad LUCA, Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine
Phd Student
Cosmin PESTEAN, Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine
Lecturer
Ciprian OBER, Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine
Lecturer
Lucia BEL, Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine
Assistant Lecturer
Andrei Razvan CODEA, Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine
Phd Student
Ileana BOGDAN, Departent of Technical and Soils Sciences, University of Agricultural Sciences and Veterinary Medicine
Professor 
Radu MANDEAL, Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine
Phd Student
Liviu OANA, Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine
Professor
Published
2017-05-18