EXPERIMENTAL STUDY REGARDING THE INFLUENCE OF PARTICULATE AIR POLLUTANTS ON OXIDANTS/ANTIOXIDANTS BALANCE

Cristina Bidian, L Fârcal, Simona Tache, R Moldovan, Nicoleta Decea

Abstract


Our study intends to explore the changes of oxidant/antioxidant (O/AO) balance generated by the exposure to particulate air pollutants (bentonite). Experiments were performed on white, male, Wistar rats, having an average weight about 150±20 g, divided in 6 experimental groups, as follows: C group (n = 10) – control group, without exposure to bentonite particle, housed in normal environmental conditions; 1st group (n = 10) – acute exposure (a single intranasal inoculation); 2nd group (n = 10) – acute exposure (a single intratracheal inoculation); 3rd group (n = 10) – chronic intranasal exposure (7 exposures); 4th group (n = 10) – chronic intranasal exposure (7 exposures), associated to vitamin E administration (7 intra peritoneal administrations), in order to evaluate the antioxidant effect of this product; 5th group (n = 10) – chronic exposure by ventilation (28 days). Acute exposure to air pollutants produced: an increase in malondialdehyde (MDA) level in serum for both groups, values being significantly higher for the 1st group as compared to control group; insignificant changes regarding carbonyl proteins (CP) level in serum for 1st group and a significant increase in the 2nd group; distinctly significant decrease in hydrogen donor (HD) ability of the serum for the 1st group and highly significant decrease for the 2nd group; highly significant decrease in thiol groups (SH) level in serum for both groups as compared to control group. Chronic exposure to air pollutants led to: a significant increase in MDA serum level regarding the 4th and the 5th group, and a distinct significant increase for the 3rd group as compared to control group; highly significant increase in CP serum level for all of three groups; highly significant decrease of the HD ability of the serum for the 3rd group and distinctly significant decrease in the 4th group and significant increase in the 5th group; insignificant decrease in SH groups serum level in the 3rd group, significant decrease in the 4th group and a distinctly significant increase for in the 5th group as compared to control group. Conclusions: 1. Particulate air pollutants caused O/AO balance disturbances, being unfavorable to AO. 2. Acute exposure determined an increase in oxidative stress parameters, especially in the group with intratracheal inoculation and a significant decrease in AO defense. 3. Chronic exposure led to a significant increase in oxidative stress markers and to a decrease in AO defense for all groups, excepting the group exposed by ventilation. 4. Chronic intranasal exposure to air pollutants associated to vitamin E administration caused different changes of the O/AO balance, namely increases in MDA and CP levels and decreases of HD and SH groups. These findings suggest the pro-oxidant effect of vitamin E in the dosage used in our experiment.

Keywords


particulate matters, oxidants, antioxidants, oxidative stress, vitamin E

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DOI: http://dx.doi.org/10.15835/buasvmcn-vm:64:1-2:2278


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