ISOLATION AND CULTIVATION OF MOUSE MESENCHYMAL STEM CELLS ON POLYPROPYLENIC SCAFFOLD
Abstract
The bone marrow represents an important source of adult mesenchymal stem cells with differentiation potential in various types of cells an tissues. The mesenchymal stem cells issued from this level dispose of a multilinear differentiation capacity. The mesenchymal stem cells were isolated on the basis of their ability to adhere to the culture dishes after 48 h. After several successive passages in order to obtain an homogenous culture; these cells were cultivated on prolypopylenic scaffold and their spontaneous diffrentiation on osteogenic line was followed. The differentiated cells were identified by means of special colorations that lead to the calcium coloration from the extracellular matri. Culture of mesenchymal stem cells on polypropylene scaffolds could offer a new solution to grafting using autologous cells from the patienta) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
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