ISOLATION AND CULTIVATION OF MOUSE MESENCHYMAL STEM CELLS ON POLYPROPYLENIC SCAFFOLD

  • Emoke Pall University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, str.Mănăştur 3-5,
  • I. Groza University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, str.Mănăştur 3-5,
  • C Tomuleasa University of Medicine and Pharmacy, Cluj-Napoca
  • Ober C University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, str.Mănăştur 3-5,
  • Daria Groza University of Medicine and Pharmacy, Cluj-Napoca
Keywords: bone marrow, mesenchymal stem cells, cultivation, polypropilenic scaffold, osteogenic differentiation.

Abstract

The bone marrow represents an important source of adult mesenchymal stem cells with differentiation potential in various types of cells an tissues. The mesenchymal stem cells issued from this level dispose of a multilinear differentiation capacity. The mesenchymal stem cells were isolated on the basis of their ability to adhere to the culture dishes after 48 h. After several successive passages in order to obtain an homogenous culture; these cells were cultivated on prolypopylenic scaffold and their spontaneous diffrentiation on osteogenic line was followed. The differentiated cells were identified by means of special colorations that lead to the calcium coloration from the extracellular matri. Culture of mesenchymal stem cells on polypropylene scaffolds could offer a new solution to grafting using autologous cells from the patient
Published
2008-11-21
Section
Research Articles