PEUCEDANUM OREOSELINUM L. MOENCH EFFECT IN EHRLICH ASCITIC CARCINOMA

B Sevastre, F. Lucian, Iulia Prodan, I. Marcus, M. Tămaş, C. Deliu

Abstract


Peucedanum oreoselinum L. Moench; (fam. Apiaceae); belongs to a large group of medicinal plants; spread around the world; used for wide range therapeutic purposes. The aerial part (Herba) was collected; from a couple of areas of Transylvania; the compounds were initially identified by chromatography; and then measured by High Performance Liquid Chromatography (HPLC). Latter a tincture 1:5 in ethylic alcohol 50°was made. After the toxicity tests; the experiment was carried out for 10 days; on 20 white Swiss male mice; each animal received; intraperitoneally 106 cells from Ehrlich ascitic carcinoma; in the first day. Experimental group received daily 0.1 ml P. oreoselinum 1:5 in ethylic alcohol 50° while control group 0.1 ml alcohol 50°. Total amount of ascitic fluid was measured; and viable tumour cell concentration was counted; production of tiobarbituric acid reactive substances (TBARS) was used to evaluate the plasma lipid peroxides levels. HPLC analysis revealed a significant content of phenols; flavonoids and coumarins; strongly related to the place of harvesting. Acute toxicity tests revealed no signs of toxicity. Ascitic Ehrlich carcinoma model provided no significant antiproliferative activity (even the ascitic volume and tumour cell concentration is lower in treated animals as compared to control; it was not statically covered); but P. oreoselinum therapy had an important antioxidant effect (p<0.05); treated animals shown lower TBARS levels (2.4±33 nmol/l) as compared to control group (6.1±1.54 nmol/l). Therefore; P. oreoselinum might be a useful tool in palliative cancer therapy

Keywords


Peucedanum oreoselinum; Ehrlich carcinoma; oxidative stress

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DOI: http://dx.doi.org/10.15835/buasvmcn-vm:65:1:384


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