An Acellular Anti-Bordetella Bronchiseptica Vaccine Efficiency Test in Wistar Rats

Attila Lehel DÉNES, Ioan MARCUS, Iulia PRODAN, Bogdan SEVASTRE, Nicodim FIŢ, George NADAŞ, Daniela CALINA

Abstract


The researches were made between February and May 2009 within the Microbiology Laboratory of the Faculty of Veterinary Medicine Cluj-Napoca. A number of 6 B. bronchiseptica free adolescent female Wistar rats were infected intranazally with a field isolate of B. bronchiseptica isolated from a pneumonic pig and the same B. bronchiseptica strain was ultrasound disintegrated for the preparation of the acellular vaccine. Three rats were subcutaneously vaccinated in days 14 and 28 after infection and in day 60 after infection all rats were euthanized through atlo-occipital dislocation under ethylic ether anaesthezia. Blood samples, lung and trachea portions were collected for bacteriological and serological tests. Clinical observations, serological and bacteriological findings showed a good protection given by the vaccine.

Keywords


Bordetella bronchiseptica; rats; serology; bacteriology; vaccine; efficiency;

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DOI: http://dx.doi.org/10.15835/buasvmcn-vm:66:1:3930


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