Development of A Hplc Method for the Determination of Isoniazid in Rat Plasma

Daniela GÂRBULEŢ, Vasile DORNEANU

Abstract


The aim of this study was development of a HPLC method for the separation and determination of isoniazid in rat plasma on a Waters e 2695 liquid chromatograph and detection in UV at 270 nm, equiped with a Luna 100-10 C18 (250 x 4.6 mm) column and a mobile phase formed by a mixture of methanol / buffer acetate pH=5.0 (20 / 80) at a flow of 1.0 ml/min. The method shows a good liniarity in the range of 0.02 – 10 µg/ml isoniazide, values of correlation coefficient r = 0.9997 .

Keywords


isoniazide; hplc; validation

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DOI: http://dx.doi.org/10.15835/buasvmcn-vm:66:1:3977




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