Assessment of the Antioxidant Potential Effect of Caffeic Acid in Retinal Pigmented Epithelial Cells

Adela Pintea

Abstract


The aim of this study was to demonstrate that caffeic acid, an antioxidant with
phenolic structure, can improve the antioxidant status of retinal pigment epithelium (RPE), when
oxidative stress is induced by high glucose concentrations in culture medium. We evaluate the high
glucose and caffeic acid cytotoxicity and their action on retinal pigmented epithelial cell viability,
intracellular reactive oxygen species generation, superoxid dismutase and gluthatione reductase
activity. Caffeic acid did not show any cytotoxic effect on RPE cells up to 250 μM in culture medium
and protect them against induced oxidation. Caffeic acid protects RPE cells by quenching the
intracellular ROS generation and by enhancing the activity of glutathione peroxidase and superoxide
dismutase. Caffeic acid treatment of cultured RPE cells can contribute to antioxidant defence in
oxidative stress conditions induced by hyperglycaemia

Keywords


caffeic acid, antioxidant, pigmented epithelial cells

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DOI: http://dx.doi.org/10.15835/buasvmcn-vm:67:1:5950




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