Use of FT-IR Technique as a Method for Differentiation of Bacterial and Fungal Strains

Adriana CRISTE, Cosmin POP, Vioara MIREŞAN, Nicodim FIŢ

Abstract


For this study we chose a series of commonly encountered bacterial and fungal microorganisms: Escherichia coli, Pseudomonas aeruginosa, Pseudomonas putida F1, Klebsiella pneumoniae, Staphylococcus aureus, Micrococcus luteus and Candida albicans. For their analysis using infrared spectrophotometry. Interpretation of data from the Spectrophotometer reading was done with the program Origin version 7. The results showed detectable differences between the spectra taken on bacteria and fungi. Could differentiate the chromatogram peaks characteristics for bacteria and fungi. The graphics made it was found that the combination of polysaccharide region (1200-900 cm-1) with “fingerprint region” (900-700 cm-1) and mixed region (1500-1200 cm-1) spectra and their derivatives primarily were most useful to characterize the FT-IR spectra, and to differentiate microorganisms. From the research done on the microorganisms: Staphylococcus aureus, Micrococcus luteus, Pseudomonas putida F1, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Candida albicans can concluded that they can differentiate using FT-IR technique. FT - IR Identification techniques is a fast and accurate of microorganisms identification techniques (organisms composition differs, molecular composition in generals is different and FT-IR spectra of them will be different) with shortening of identification time. Method FT - IR can be used for rapid identification of microorganisms. Being only need a small amount of culture for 24 hours and 5 minutes to read Spectrophotometer IR light.

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DOI: http://dx.doi.org/10.15835/buasvmcn-vm:1:68:6745


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