Molecular Detection of Pasteurella multocida Strains from Oral Swabs in Pets

Simona IVANA, Viorica CHIURCIU, Dana Magdalena CAPLAN, Camelia BALA, Lucian IONIŢĂ, Carmen IONIŢĂ, Iudith IPATE, Cristina PÂRVU, Alexandru POPESCU

Abstract


The molecular detection by means of the polymerase chain reaction (PCR) represents a much more sensitive, specific and fast detection method of Pasteurella and confirms the infection. Although the isolation and identification of the pathogenic agent represent the most reliable method of demonstration of its existence, the results of the bacteriologic examination are frequently false negative, owing to the fact that P. multodica is either rather easily destroyed during the transportation or eliminated from the culture medium by the bacteriological suprainfection germs (nasal flora or contaminants).Pasteurellae were identified both in clinically healthy cats and in ill cats. These dates suggest that the contaminated cats, regardless of their clinical state, may transmit de bacteria to humans by bite or scratch. In this work, cats kept as pets from Bucharest were studied with suspect to their contamination with Pasteurella. Of 44 samples taken from the oral cavity of cats, 22 Pasteurella spp. strains could be isolated, 18 of which belonged to the Pasteurella multocida. The organisms were isolated both from healthy and from infected cats.

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DOI: http://dx.doi.org/10.15835/buasvmcn-vm:1:68:6762


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