Immunohistochemical Expression of Melan-A in Canine Melanic Tumors

Raouad MOUSSA, Bogdan SEVASTRE, Marian TAULESCU, Pompei BOLFĂ, Adrian GAL, Flaviu TABARAN, Andras NAGY, Cosmina CUC, Gabriel BORZA, Cornel CATOI


We evaluated the expression of Melan A/MART-1 (melanoma antigen
recognized by T cells). Material and methods: 12 clinical specimens of dog melanic tumors
were diagnosed with Melan-A by immunohistichemmical method. Results: Melan-A
reactivity was demonstrated in (10-12, 83.3%) melanoma, one metastatic melanoma and one
melanocytoma were positive. In canine cutaneous malignant melanoma, the intensity of
staining was high in 6 cases (50 %), over moderate in 1 case (8.3%), moderate in 3 cases
(25%) weak in 0 case (0%), and absent in 2 cases (16.6%), but the staining was over moderate
in the metastatic melanoma (59.27%) and in melanocytoma (67.49%). Nuclear staining was
visible in 0 canine melanoma case, and was staining visible in some cells and invisible in
others in 6 melanoma cases, one metastatic melanoma and one melanocytoma case but was no
visible in 4 melanoma cases. Spindle cells were in general over moderate stain in (3/3) one of
them was melanocytoma. One balloon melanoma type cells was a high stain with Melan-A.
Conclusion: The percentages of marker stain were between (40.43%- 97.89%), Melan-A
reactivity was staining in majority cases. The intensity of Melan-A stain was between
moderate to intense in melanoma, while in melanocytoma and metastatic melanoma cases
were over-moderate. The staining in majority cases was heterogeneous. All tumor cell types
demonstrated reactivity for Melan A. In all tumoural cells of cases were reacted diffusely in
their cytoplasm with Melan-A,

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