Immunohistochemical Quantification of the Tumor Necrosis Factor (TNF) Receptor II Expression in the Hepatic Tissue after Systemic Administration of the DNA-SWCNT

Flaviu TABARAN, Cornel CĂTOI, Simona CLICHICI, Teodora MOCANU, Alexandru BIRIS, Stefania SIMON, Andras NAGY, Marian TAULESCU, Pompei BOLFĂ


The Tumor Necrosis Factor is a key mediator in hepatic inflammatory response during acute exposure to xenobiotics. The cellular effects of TNF are mediated via two cell surface receptors, TNF receptor 1 and TNF receptor 2. The purpose of this study was to examine the expression and to identify the cellular localization of TNFR2 in hepatic tissue by immunohistochemistry after systemic administration of the SWCNT. In the same time the cellular infiltration and the weight of the liver was correlated with the TNF alpha receptor 2 expression. Mice were exposed intraperitoneally (ip) to either vehicle, phosphate-buffered saline (PBS), or SWCNT-DNA (1.5ml, 2.925 mg/kg) for 48 h. The hepatic response associated with SWCNT systemic administration was characterized by increased expression of the TNF receptor II especially in the hepatocytes from the centrilobular and midzonal areas of hepatic lobule. This induced elevation of the TNF receptor II is not followed by hepatic necrosis, inflammatory infiltration or significant changes in liver weight.

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