Histopathological Study on the Influence of AD DIEE Biophytomodulators on Cartilage Regeneration in a Leporidae Model

Cristian CRECAN, Daniela OROS, Ancu DINCA, Flaviu TABARAN, Liviu OANA


Abstract. Articular cartilage regeneration was and is one of the most controversial topics in human and veterinary orthopedic surgery research. Studies on vascularization and joint nutrition represented crucial steps in this research. The purpose of this study is the histological evaluation of articular cartilage regeneration using AD-DIEE biophytomodulators in surgically induced chondral defects. Identical chondral defects were created in 33 common breed rabbits of both genders, aged 8 months. The test subjects were divided into 4 groups: a group that was treated with AD-DIEE, biophytomodulators; a reference group left untreated and 2 reference groups treated with hyaluronic acid which represents the standard treatment at this moment. Cartilage samples for histological study were collected 14, 27 and 60 days post-operatory, prepared with Hematoxylin-Eosin and examined with an optical microscope. Results showed a fibrous tissue of the cartilage defect in every one of the samples, conjunctive fibrous tissue in different developmental stages that filled up the defect’s cavities. We have found more advanced healing processes in the group treated with biophytomodulators as compared to the untreated reference group, a better chondrocytic organization, the lack of chondral fissures as well as chondral matrix normochromia.


rabbits, biophytomodulators, cartilage, hyaluronic acid

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DOI: http://dx.doi.org/10.15835/buasvmcn-vm:71:1:9629

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