The effect of overpopulation on Neutrophile/Lymphocyte ratio and WBC values, in normal and Propranolol treated rats

G COTOR, G GAJAILA, Mimi DOBREA, Iuliana GAJAILA

Abstract


Our study evaluate the effects of the overpopulation stress on some leucogram parameters in normal and Propranolol treated rats. In this purpose 18 adult Wistar rats were separated in standard cages, for 6 days, in 3 experimental groups,. The density of rats varies: Group I = 3 rats/cage; Group II = 6 rats/cage; Group III = 6 rats/cage. The rats belonging to the Group III were injected with Propranolol (5 mg/kg, s.c.). In the 6th day of the experiment blood samples were collected. The WBC count and N/L ratio were determined. The WBC values were significantly increased (P < 0.05) in Group II, compared to Group I (6.77 %) and III (7.71%). This leukocytosis was an adaptive response to overpopulation stress. These results suggest that in this response is involved predominantly the sympatho-adrenal mechanism. The N/L ration were significantly increased (P < 0.05) in Group II, compared to Group I (16.94 %). This parameter was increased in Group II, compared to Group III (6.15%). But this increase was not significant, statistically speaking. According to our results the overpopulation in rat seems to be a very stressful factor. Given that Propranolol had a weak effect on N/L ratio, means that, in this case, the sympatho-adrenal mechanism is not the only involved mechanism.


Keywords


overpopulation stress; leukocyte; N/L ratio; rat.

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DOI: http://dx.doi.org/10.15835/buasvmcn-vm:71:1:9672




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