Evaluation of Innocuity of Isoflurane in Rat Lung and Liver
Abstract
The aim of the present study was to assess the innocuity of isoflurane in rat lung and liver through histopathological examination. Experimental protocol: 20 female Wistar rats were divided intro 4 groups, each consisting of 5 individuals: control group, group I, II and III. Female rats from the control group were not exposed to any anesthetic treatment, while female rats from the other groups (I, II and III) were exposed to isoflurane three times, for 2 hours, every other day. Animals from the control group and group I were sacrificed immediately after the last exposure (0 h). Group II was sacrificed 6 hours after the last exposure and group III 24 hours after the last exposure. Fragments of the lung and liver were collected from the 20 female rats taken into study and then histologically processed (fixed in 10% buffered formalin and embedded in paraffin). After obtaining 5 μm thick sections, they were stained using Goldner’s Trichrome method. Histopathological examination of the tissue specimens showed no structural changes perceptible through light microscopy, thus showing that isoflurane did not exert a toxic action in these two organs. The present study proved a good innocuity of isoflurane in rat lung and liver.
Authors who publish with this journal agree to the following terms:a) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
