CONVERSION OF RAPD MARKERS INTO SCAR MARKERS FOR A STRAIGHTFORWARD DIAGNOSIS OF POTATO INFECTION WITH PHYTOPHTHORA INFESTANS

C. Botez, D. Pamfil, I. Gotea, Meda Lucaci, Erika Balazs, A. Taoutaou, P. Raica

Abstract


In our researches we tried to convert the P.infestans RAPD markers into SCAR markers for a more operative diagnosis of potato late blight in the infected material. For this goal, several monomorphic and polymorphic RAPD sequences were recuperated from the agarose gel, and cloned into E.coli bacteria. The cloned monomorphic sequences were sequenced, establishing the nucleotide sequence. Relying on this several pair of primers was designed. One pair from them, with the following nucleotide sequences AGC CCA CAC TGA AAT TAC GC/GGC TAC CGG ATC AGT ACC AA), gave amplification product only with P.infestans DNA. Being specific, this pair pf primer could be used for a straightforward potato late blight diagnosis from the infected material, and our objective concerning the conversion of P.infestans RAPD markers into SCAR markers could be considered accomplished.

Keywords


molecular polymorphism; cloning RAPD markers; DNA sequencing; primer design

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DOI: http://dx.doi.org/10.15835/buasvmcn-asb:65:1-2:1111




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