Analyses of the Chemical Composition of the Extracts of Bee Brood and Adult Bees

Maria Pojar-Fenesan, Ana Balea, Irina Ciotlaus, Mirela Miclean


Living organisms in the beehive communicate by chemical language. The study of the chemical composition of bee brood extracts and adult bees is interesting assuming as bee brood emissions could be “words†in the “dialogues†in the hive.This study proposes an analysis of the composition of worker bee brood and drone brood extracts, using different solvents. The bioassays start with GC-MS analysis of various extracts obtained by dissolving biological material (bee brood, adult bees) in different solvents (ethylic ether, hexane, pentane, aqueous solution, brine). The substances found in these extracts that could be kairomones are fatty acids (9-Octadecenoic acid and Hexadecanoic acid) and methyl ester fatty acid, 2-methyl propionic acid, hydrocarbons with long chain (Heptacosane, Octacosane). “Communication†by some of these substances will be used for the behavioural studies of the bees’ enemy mite, Varroa destructor.


Bee brood, GC-MS, semiochemicals

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