The Role of Nitrobenzoic Acid Derivatives on Callus Induction and Plant Regeneration in Paulownia shan tong

Roxana Luca, Manuela Crișan, Dorica Botău


Paulownia is a multipurpose tree species with a rapid growth which is cultivated in several temperate zones worldwide for its economic importance. The present study was undertaken to investigate the effects of different alkanolamine salts of 4-nitrobenzoic acid on in vitro callus induction and plant regenerating in Paulownia shan tong.The alkanolamine salts, namely methylethanolamine 4-nitrobenzoate (4-NO2BA MMEA), diethanolamine 4-nitrobenzoate (4-NO2BA DEA) and triethanolamine 4-nitrobenzoate (4-NO2BA TEA) are new synthesized compounds with biological activity and low toxicity. The influence of title compounds on sprout length, number of internodes, number of leaves and number of ramifications has been studied at two different concentrations: 1 mg/L respectively 0.5 mg/L. The experimental data show that new tested compounds have a better growth effect on plant vegetable parties in report to control, the most eloquent results being obtained for 4-NO2BA MMEA. For callus induction 2,4-D hormonal balances were attempted and the development of callus was examined at two different concentrations. An induced callus development was observed regarding callus dimensions for all explants inoculated on culture medium supplemented with 2,4-D.


alkanolamine salts of 4-nitrobenzoic acid; callus; in vitro culture; Paulownia

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