Nigella Sativa and Oriental Spices with Protective Role in Iron Intoxication: in vivo Experiments on Rabbits

Mirela Ahmadi, Dorel Dronca, Gianina Dodi, Cornelia Milovanov, Dinu Gavojdian, Cristian Dumitru, Mihaela Scurtu


Homeostasis of hematological parameters is essential for assuring a general health status for any living organism. Iron is one of the essential mineral, involved in many vital processes - mainly in blood cells production, but in the same way it can become toxic in very high concentration. Hemoglobin and red blood cells are directed related with the iron ion, due to the high quantity (70%) of total iron from organism being part of the blood (hemoglobin) and muscle (myoglobin) cells. Ferrous ion is part of hemoglobin structure, and red blood cells. But, the administration of high doses of iron can negatively affect the general health status, because the iron alters the enzymatic system in the vital organs. The aim of our experimental study was to verify the hypothesis that in rabbit’s organism, after intraperitoneal administration of 15g Fe2+/body weight as ferrous-gluconate hydro solution, a special diet based on a complex, fresh, organic vegetables (roots and leaves) protects the organism by iron intoxication and help the hematological homeostasis. The research experiment was conducted during 43 days in summer time, on German Lop Eared breed young rabbits, which were protected with a diet that consisted of administration of Nigella sativa, some oriental spices (Allium ampeloprasum, Allium tuberosum, Coriandrum sativum, Eruca sativa, Cucumis sativus, Raphanus sativus, Trigonella foenum-graecum) and other vegetables (Trifolium, Petroselinum crispum, Dacus carrota subsp.sativus and Cucumis sativus). At the final of experiment we collected blood samples for hematological test and we evaluated the erythrocytes, leukocytes, platelets, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and red cell distribution width. The results were analytical evaluated and only for hemoglobin we obtained significant increase value in experimental rabbits compared to control group of rabbits.


iron intoxication, Nigella sativa, oriental spices, rabbits

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