UV-INDUCED CYCLOBUTYL-PYRIMIDINE-DIMERS AND THEIR PHOTOREPAIR. IN ROMANIAN CULTIVARS OF BEAN (PHASEOLUS VULGARIS L.).

Abstract

This review summarizes the early effects and responses of low doses of UV-B at the molecular level. in the case of four bean cultivars from Romania. The DNA molecules of the plant cells are damaged by UV due to the formation of different photoproducts. such as cyclobutane - pyrimidine dimers. (CPDs). which in turn can be combated by specialized photoreactivating enzyme systems. The investigation were done by using ELISA with specific monoclonal antibodies. CPDs formation increased during UV-B exposure. and photorepair led to descrease.
The choice of Phaseolus vulgaris as biological material for investigations. can be explained by the importance of seeds and legumes in humans nutrition. being considered since centuries the poor peoples “meat” because of the increased level of high quality proteins. and the high energetically level. and due to the importance in soil amelioration. being used as very good previous culture plant for many other species.