AFLP AS A MODERN TECHNIQUE FOR DNA FINGERPRINTING AND IDENTIFICATION TULIPA CULTIVARS

Ioana Olivia Bondrea, Doru Pamfil, Sjaak van Heusden, Jaap van Tuyl, Fier Meijer Dekens, Mircea Bondrea, Radu Sestras, Anda Raluca Rusu, Meda Lucaci, Bianca Ionela Patrascu, Valentin A. Balteanu

Abstract


The recently introduced PCR- based DNA fingerprinting technique AFLP (Amplified Fragment Length Polymorphism ) allows the selective amplification of subsets of genomic restriction fragments. AFLP has been used for multiple purposes such as construction of linkage maps. marker saturation at specific genomic regions. analysis of genetic diversity and molecular phylogeny and cultivar identification. AFLP can be tailored by varying the number of selective nucleotides added to core primers and can allow accurate amplification. even in complex template mixtures generated from plant species with very large genomes. In this study Tulipa. a plant species with a very large genome (12 chromosomes) was tested for adapting the AFLP protocol. The results indicated that obtain reproductible AFLP patterns. clear and labor saving fingerprints when seven selective nucleotides were used.

Keywords


AFLP genetic marker; Tulipa genus;DNA fingerprinting; PCR; preamplification; selective nucleotides

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DOI: http://dx.doi.org/10.15835/buasvmcn-asb:64:1-2:1931


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