XANTOPHYILL ESTERS: ISOLATION. SEPARATION AND ENZYMATIC HYDROLYSIS

ANDREEA BUNEA, ADELA PINTEA, SANDA ANDREI, AUGUSTA LUJERDEAN, CARMEN SOCACIU

Abstract


Carotenoids are natural pigments. produced by higher plants. some bacteria and algae with important antioxidant properties. Carotenoid xanthophylls are found in higher plants in esterified form. The fatty acids which esterified the carotenoids are mainly saturated fatty acids. but in some plants unsaturated fatty acids can also be present in low amounts [Breithaupt and Bamedi. 2001].The highest carotenol esters content is usually associated with senescence and fruit ripening [Britton. 1995; Minguez-Mosquera et al.. 1994]. Lipase from Candida rugosa is a universal lipolytic biocatalyst and cleaves carotenoid esters with high yields [Liu et al.. 1998; Breithaupt. 2000]. In this study we separate de carotenoid esters from three plants: Physalis alkekengi L.. Tagetes erecta and Seabuckthorne berries; we used lipases from Candida rugosa and from porcine pancreas and we compared the yield of hydrolysis of ?-cryptoxanthin esters separated from Physalis alkekengi L. sepals. HPLC-PDA was used for separation and quantification of carotenoid esters and for monitoring the ester hydrolysis.
It can be observed that in the case of enzymatic hydrolysis of ?-cryptoxanthin esters. porcine lipases has lowest efficiency than lipases from Candida rugosa. The yield of enzymatic hydrolysis with porcine lipases is not very different after 24 hours (81 % respectively 71 %).

Keywords


carotenoid esters; lipases. HPLC

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DOI: http://dx.doi.org/10.15835/buasvmcn-asb:64:1-2:1936




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