DNA EXTRACTION AND QUANTIFICATION OF DIPLOID AND TETRAPLOID RED CLOVER (TRIFOLIUM PRATENSE L.) FROM LEAF TISSUE USING KIT EXTRACTION (SIGMA)

MARIOARA URDA, M. SAVATTI

Abstract


This paper describes the process of DNA extraction and quantification of diploid and tetraploid red clover (Trifolium pratense L.). Red clover is an important forage crop for research purposes which has seen a gradual decline in use for a century. The paper begins by describing the advantages of red clover and arguing that its use may become important once again within the context of sustainable agriculture. The process of DNA extraction using a commercial extraction kit is then described in detail. The results section considers DNA quantification using both spectrophotometer and electrophoresis processes. Quantification is used to determine the concentration and purity of the laboratory samples of red clover used in the research laboratory

Keywords


DNA extraction; red clover; leaf

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DOI: http://dx.doi.org/10.15835/buasvmcn-asb:64:1-2:2189




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